Thermo Scientific™ Maleimide-Activated Blue Carrier™ Protein
Make hapten-carrier immunogens comprising cysteine-containing peptides and other thiolated antigens with sulfhydryl-reactive immunogenic hemocyanin (CCH).
Manufacturer: Thermo Scientific™ 77664
Thermo Scientific™ Maleimide-Activated Blue Carrier Protein is sulfhydryl-reactive immunogenic hemocyanin (CCH) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.
Blue Carrier Protein is a large homocyanin that is highly immunogenic like keyhole limpet hemocyanin (KLH) yet is more soluble. The carrier is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens. Numerous antigen molecules can be conjugated to each Blue Carrier Protein molecule to make them highly immunogenic and increase the likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest.
Optimized activation – specifications require that each lot is activated with more than 400 moles of sulhydryl-reactive maleimide per mole of carrier, ensuring consistently high levels of conjugatio
Sulfhydryl-reactive – engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production
Simple, rapid procedure – complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals
High-quality carrier protein – Blue Carrier Protein is ultrapure Concholepas concholepas hemocyanin (CCH) prepared from molluscan hemolymph
Highly immunogenic and soluble – CCH has a high molecular mass that elicits a stronger immune response than BSA or ovalbumin
|Imject Maleimide-Activated Blue Carrier Protein|
|CCH, Concholepas concholepas hemocyanin|
|Activated protein lyophilized in 10mM sodium phosphate, 150mM sodium chloride, 10mM EDTA, 50mM sucrose, pH 7.2|
|5 conjugation reactions, each with up to 2mg sulfhydryl-containing peptide|