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Ly-6G Monoclonal Antibody (1A8-Ly6g), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Supplier: Invitrogen 46966880
Description
Description: The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G, which is also known as Gr-1. Ly-6G is a 25-kDa GPI protein expressed exclusively by neutrophils. Studies suggest an important role in neutrophil infiltration, recruitment and migration. Ly-6G associates with B2 integrins CD11a and CD11b and can attenuate B2 integrin expression and function. The ligand for Ly-6G is currently unknown. Unlike the RB6-8C5 antibody, 1A8-Ly6g is reported to only bind to Ly-6G found on neutrophils. Moreover, 1A8-Ly6g has been used to deplete neutrophils and reported to have antagonist activity. Applications Reported: This 1A8-Ly6g antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1A8-Ly6g antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative.
Ly-6G Monoclonal antibody specifically detects Ly-6G in Mouse samples. It is validated for Flow Cytometry.Specifications
Ly-6G | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P35461 | |
Ly6g | |
Affinity chromatography | |
RUO | |
546644 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
1A8-Ly6g | |
PerCP-eFluor 710 | |
Ly6g | |
Gr1; Gr-1; Ly6g; Ly-6G; ly-6G.1; lymphocyte antigen 6 complex, locus G; lymphocyte antigen 6G | |
Rat | |
25 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.