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Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), APC-eFluor™ 780, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Supplier: Invitrogen 47593182
Description
Description: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations. eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4). It is recommended that 1A8-Ly6G (cat. 9668) be used when looking at Ly-6G specific targets. Applications Reported: This RB6-8C5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RB6-8C5 antibody has been tested by flow cystometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm).
Ly-6G/Ly-6C Monoclonal antibody specifically detects Ly-6G/Ly-6C in Mouse samples. It is validated for Flow Cytometry.Specifications
Ly-6G/Ly-6C | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P0CW02, P0CW03, P35461 | |
Ly6c1, Ly6c2, Ly6g | |
Affinity chromatography | |
RUO | |
100041546, 17067, 546644 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
RB6-8C5 | |
APC-eFluor 780 | |
Ly6g | |
AA682074; AA959465; Gr1; Gr-1; Granulocyte Marker; Ly6c; Ly-6C; Ly-6C.2; Ly6c1; Ly-6C1; Ly6c2; Ly-6C2; Ly6g; Ly-6G; ly-6G.1; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6 complex, locus C1; lymphocyte antigen 6 complex, locus C2; lymphocyte antigen 6 complex, locus G; lymphocyte antigen 6C precursor (Ly-6C); lymphocyte antigen 6C1; Lymphocyte antigen 6C2; lymphocyte antigen 6G; Lymphocyte antigen Ly-6C; lymphocyte differentiation antigen; PML; PMN | |
Rat | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2b κ |
For Research Use Only.