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Ly-6A/E (Sca-1) Rat anti-Mouse, PE-eFluor® 610, Clone: D7, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS61598182

Catalog No. 501124861



Description

Description

Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peri

We recommend using a 610/20 band pass filter (equivalent to PE- Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. Ly-6A/E molecules are implicated in the regulation of T cell activation.
Specifications

Specifications

Ly-6A/E (Sca-1)
Monoclonal
0.2 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q64253, P05533
lymphocyte antigen 6 complex, locus A/E
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
D7
PE-eFluor® 610
Ly6e
Liquid
Ly6a, Ly6e
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
110454, 17069
Documents

For Research Use Only.