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Ly-6A/E (Sca-1) Rat anti-Mouse, APC, Clone: D7, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS17598182

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Catalog No. 501129396



Description

Description

Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset. Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This D7 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Ly-6A/E molecules are implicated in the regulation of T cell activation.
Specifications

Specifications

Ly-6A/E (Sca-1)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q64253, P05533
lymphocyte antigen 6 complex, locus A/E
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
D7
APC
Ly6e
Liquid
Ly6a, Ly6e
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
110454, 17069
Documents

For Research Use Only.