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Thermo Scientific™ Luminaris™ Color Probe qPCR Master Mixes

Optimize qPCR with probe detection using standard cycling protocols with these ready-to-use master mixes with premixed UDG, available with or without blue color.

$217.35 - $2,546.25

Specifications

For Use With (Application) Gene expression analysis; siRNA validation; Genotyping; Pathogen detection
Includes Luminaris Color Probe qPCR Master Mixes include Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; 40X Yellow Sample Buffer with a yellow dye; Nuclease-free water
Storage Requirements Note that ROX dye is light sensitive. Exposure to light should be minimized
View More Specs
Products
Catalog Number Mfr. No. Price Quantity    

FERK0353

 
thermo scientific™
K0353
Each for $915.60

FERK0351

 
thermo scientific™
K0351
Each for $217.35

FERK0331

 
thermo scientific™
K0331
Each for $217.35

FERK0343

 
thermo scientific™
K0343
Each for $915.60

FERK0344

 
thermo scientific™
K0344
Each for $2,546.25

FERK0354

 
thermo scientific™
K0354
Each for $2,546.25

FERK0342

 
thermo scientific™
K0342
Each for $399.00

FERK0333

 
thermo scientific™
K0333
Each for $915.60

FERK0341

 
thermo scientific™
K0341
Each for $217.35

FERK0332

 
thermo scientific™
K0332
Each for $399.00

FERK0334

 
thermo scientific™
K0334
Each for $2,546.25

FERK0352

 
thermo scientific™
K0352
Each for $399.00
Description & Specifications

Specifications

For Use With (Application) Gene expression analysis; siRNA validation; Genotyping; Pathogen detection
Includes Luminaris Color Probe qPCR Master Mixes include Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; 40X Yellow Sample Buffer with a yellow dye; Nuclease-free water
Storage Requirements Note that ROX dye is light sensitive. Exposure to light should be minimized

Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control. The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms.

Highlights

  • Blue Master Mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Excellent qPCR performance
    • Specificity — Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
    • Sensitivitity — single copy detection
    • Wide linear range — accurate quantification across 9 orders of magnitude
    • Reproducibility and convenience — ready-to-use 2X master mix
    • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX for different real-time platforms
  • Standard cycling protocols