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Thermo Scientific™ Luminaris™ Color HiGreen qPCR Master Mixes

Optimize qPCR with SYBR Green using standard cycling protocols with these ready-to-use master mixes with premixed UDG, available with or without blue color.

$213.15 - $2,520.00

Specifications

For Use With (Application) Gene expression analysis; siRNA validation; Genotyping; Pathogen detection
Includes Hot Start Taq DNA Polymerase, UDG, and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; 40X Yellow Sample Buffer with a yellow dye; Nuclease-free water
Storage Requirements Note that SYBR Green and ROX dyes are light sensitive. Exposure to light should be minimized.
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Products
Catalog Number Mfr. No. Price Quantity    

FERK0393

 
thermo scientific™
K0393
Each for $905.10

FERK0363

 
thermo scientific™
K0363
Each for $905.10

FERK0391

 
thermo scientific™
K0391
Each for $213.15

FERK0374

 
thermo scientific™
K0374
Each for $2,520.00

FERK0373

 
thermo scientific™
K0373
Each for $905.10

FERK0392

 
thermo scientific™
K0392
Each for $393.75

FERK0364

 
thermo scientific™
K0364
Each for $2,520.00

FERK0372

 
thermo scientific™
K0372
Each for $393.75

FERK0381

 
thermo scientific™
K0381
Each for $213.15

FERK0362

 
thermo scientific™
K0362
Each for $393.75

FERK0394

 
thermo scientific™
K0394
Each for $2,520.00

FERK0361

 
thermo scientific™
K0361
Each for $213.15

FERK0383

 
thermo scientific™
K0383
Each for $905.10

FERK0371

 
thermo scientific™
K0371
Each for $213.15

FERK0384

 
thermo scientific™
K0384
Each for $2,520.00

FERK0382

 
thermo scientific™
K0382
Each for $393.75
Description & Specifications

Specifications

For Use With (Application) Gene expression analysis; siRNA validation; Genotyping; Pathogen detection
Includes Hot Start Taq DNA Polymerase, UDG, and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; 40X Yellow Sample Buffer with a yellow dye; Nuclease-free water
Storage Requirements Note that SYBR Green and ROX dyes are light sensitive. Exposure to light should be minimized.

Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

  • Blue master mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Excellent qPCR performance:
    • Specificity -Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
    • Sensitivity -single copy detection
    • Wide linear range -accurate quantification across 9 orders of magnitude
    • Reproducibility and convenience -ready-to-use 2X master mix
    • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols