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Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.
Supplier: Thermo Scientific™ K0391
Includes: Luminaris Color HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye; 40X Yellow Sample Buffer; Nuclease-Free Water
Description
Thermo Scientific™ Luminaris Color HiGreen Master Mixes are ready-to-use qPCR master mixes with color and premixed UDG optimized for SYBR Green chemistry using standard cycling protocols.
- Blue master mix and Yellow Sample Buffer for easy pipetting
- UDG in the master mix to prevent carry-over contamination
- Equal performance of blue and colorless master mixes
- Versions with premixed ROX or fluorescein for different real-time platforms
- Standard cycling protocols
- Excellent qPCR performance
- Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Sensitivity: single copy detection
- Wide linear range: accurate quantification across 9 orders of magnitude
- Reproducibility and convenience: ready-to-use 2X master mix
Specifications
| Contains: • 2 x 1.25 mL Luminaris Color HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye; sufficient for 250 x 20 μL reactions • 1 x 1.25 mL 40X Yellow Sample Buffer • 2 x 1.25 mL Nuclease-Free Water Store at -20°C. |
|
| High | |
| Taq DNA Polymerase | |
| BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene | |
| qPCR Master Mix | |
| Gene Expression | |
| 2 X | |
| DNA, dsDNA |
| SYBR | |
| qPCR | |
| Standard | |
| Luminaris | |
| 250 Reactions | |
| 2X | |
| 250 Reactions |
For Research Use Only. Not for use in diagnostic procedures.