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Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, Fluorescein

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Manufacturer:  Thermo Scientific™ K0382

 View more versions of this product

Catalog No. FERK0382


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes:
  • Luminaris Color HiGreen Fluorescein qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye. The master mix contains fluorescein passive reference dye for spe

Specifications

Description

PCR Reagent, Real-Time; Luminaris Color HiGreen qPCR Master Mix, Fluorescein; 500 Reactions; For use with: BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5; Sample type: DNA, dsDNA

  • Blue master mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: single copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix
Description & Specifications

Specifications

Detection Method: SYBR, PCR Method: qPCR
500 Reactions
Gene expression analysis; siRNA validation; Genotyping; Pathogen detection
500
Note that SYBR Green and ROX dyes are light sensitive. Exposure to light should be minimized.
BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5
DNA, dsDNA
Hot Start Taq DNA Polymerase, UDG, and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; 40X Yellow Sample Buffer with a yellow dye; Nuclease-free water
500 x 20µL rxns