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Thermo Scientific™ Luminaris™ Color HiGreen qPCR Master Mixes


Optimize qPCR with SYBR Green using standard cycling protocols with these ready-to-use master mixes with premixed UDG, available with or without blue color.

Manufacturer: thermo scientific™  K0381

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Catalog No. FERK0381

  • / Each
 

Description & Specifications

Specifications

For Use With (Application) Gene expression analysis; siRNA validation; Genotyping; Pathogen detection
Includes Hot Start Taq DNA Polymerase, UDG, and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; 40X Yellow Sample Buffer with a yellow dye; Nuclease-free water
No. of Reactions 250
Quantity 250 x 20µL rxns
Storage Requirements Note that SYBR Green and ROX dyes are light sensitive. Exposure to light should be minimized.

Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Thermo Scientific Luminaris Color HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms.

  • Specificity Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers

  • Sensitivity detects low copy number targets

  • Wide linear range accurate quantification across 9 orders of magnitude

  • Reproducibility and convenience ready-to-use 2X master mix

  • Blue master mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Luminaris Color HiGreen High ROX qPCR Master Mix (2X) and Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) contain different amount of ROX passive reference dye for specific real-time platforms
  • Luminaris Color HiGreen Fluorescein qPCR Master Mix (2X) contains fluorescein passive reference dye for specific real-time platforms (see Real-time PCR instrument compatibility chart)

Includes:

  • Hot Start Taq DNA Polymerase, UDG, and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye

    40X Yellow Sample Buffer with a yellow dye

    Nuclease-free water

Recommended for:

Gene expression analysis; siRNA validation; Genotyping; Pathogen detection