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Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, Fluorescein

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Manufacturer:  Thermo Scientific™ K0381

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Catalog No. FERK0381



For Research Use Only. Not for use in diagnostic procedures.

  • Luminaris Color HiGreen Fluorescein qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye. The master mix contains fluorescein passive reference dye for spe



PCR Reagent, Real-Time; Luminaris Color HiGreen qPCR Master Mix, Fluorescein; 250 Reactions; For use with: BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5; Sample type: DNA, dsDNA

  • Blue master mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: single copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix
Description & Specifications


Detection Method: SYBR, PCR Method: qPCR
BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5
Hot Start Taq DNA Polymerase, UDG, and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; 40X Yellow Sample Buffer with a yellow dye; Nuclease-free water
250 x 20µL rxns
Real time PCR (qPCR), gene expression analysis, siRNA validation, genotyping and pathogen detection
250 Reactions
Store at -20°C.