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Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, Low ROX

Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Manufacturer:  Thermo Scientific™ K0373

 View more versions of this product

Catalog No. FERK0373


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes:
  • Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dy

Specifications

Description

PCR Reagent, Real-Time; Luminaris Color HiGreen qPCR Master Mix, Low ROX; 1250 Reactions; For use with: 7500 System, QuantStudio, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, ViiA 7 System; Sample type: DNA, dsDNA

  • Blue master mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: single copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix
Description & Specifications

Specifications

Detection Method: SYBR, PCR Method: qPCR
7500 System, QuantStudio™, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, ViiA™ 7 System
DNA, dsDNA
1250
Note that SYBR Green and ROX dyes are light sensitive. Exposure to light should be minimized.
Real time PCR (qPCR), gene expression analysis, siRNA validation, genotyping, pathogen detection
1250 Reactions
Hot Start Taq DNA Polymerase, UDG, and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; 40X Yellow Sample Buffer with a yellow dye; Nuclease-free water
1250 x 20µL rxns