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Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, Low ROX

Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Manufacturer:  Thermo Scientific™ K0371

 View more versions of this product

Catalog No. FERK0371


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes:
  • Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dy

Specifications

Description

PCR Reagent, Real-Time; Luminaris Color HiGreen qPCR Master Mix, Low ROX; 250 Reactions; For use with: 7500 System, QuantStudio, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, ViiA 7 System; Sample type: DNA, dsDNA

  • Blue master mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: single copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix
Description & Specifications

Specifications

Detection Method: SYBR, PCR Method: qPCR
Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms and is supplied with 40X Yellow Sample Buffer and nuclease-free water.
DNA, dsDNA
250
Note that SYBR Green and ROX dyes are light sensitive. Exposure to light should be minimized.
7500 System, QuantStudio™, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, ViiA™ 7 System
250 Reactions
Gene expression analysis; siRNA validation; Genotyping; Pathogen detection
250 x 20µL rxns