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Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, High ROX

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Manufacturer:  Thermo Scientific™ K0363

 View more versions of this product

Catalog No. FERK0363


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes:
  • Luminaris Color HiGreen High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye. The master mix contains high concentration of ROX passive reference

Specifications

Description

PCR Reagent, Real-Time; Luminaris Color HiGreen qPCR Master Mix, High ROX; 1250 Reactions; For use with: 7000 System, 7300 System, 7700 System, 7900HT System, StepOne System, StepOnePlus System; Sample type: DNA, dsDNA

  • Blue master mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: single copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix
Description & Specifications

Specifications

Detection Method: SYBR, PCR Method: qPCR
7000 System, 7300 System, 7700 System, 7900HT System, StepOne™ System, StepOnePlus™ System
DNA, dsDNA
1250
Note that SYBR Green and ROX dyes are light sensitive. Exposure to light should be minimized.
Real time PCR (qPCR), gene expression analysis, siRNA validation, genotyping and pathogen detection
1250 Reactions
Hot Start Taq DNA Polymerase, UDG, and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; 40X Yellow Sample Buffer with a yellow dye; Nuclease-free water
1250 x 20µL rxns