LTB4 Parameter Assay Kit, R&D Systems
a 4.5 hour forward sequential competitive enzyme immunoassay designed to measure LTB4 in cell culture supernates, serum, and plasma
Manufacturer: R & D Systems RDSKGE006B
Kit, ELISA; R&D Systems; LTB4 Parameter Assay Kit; One 96-well plate; 1 Kit; Sufficient materials to run ELISAs on at least one 96-well plateThis assay is based on the forward sequential competitive binding technique in which LTB4 present in a sample competes with a fixed amount of horseradish peroxidase (HRP)-labeled LTB4 for sites on a chicken polyclonal antibody. During the incubations, the chicken polyclonal antibody becomes bound to the rabbit anti-chicken antibody coated onto the microplate. Following a wash to remove excess conjugate and unbound sample, a substrate solution is added to the wells to determine the bound enzyme activity. Following color development, the assay is stopped, and the absorbance is read at 450 nm. The intensity of the color is inversely proportional to the concentration of LTB4 in the sample.
|Cross-species reactivity not tested. <0.5% cross-reactivity observed with available related molecules.|
|1 x 96-well plate|