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Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit

Study RNA-protein interactions with this non-radioactive EMSA kit for gel shift assays.

Manufacturer:  Thermo Scientific™ PI20158

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Catalog No. PI20158



Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.


  • Sensitive – chemiluminescent detection comparable to radioactive detection

  • Time-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems

  • Flexible – compatible with RNA probes biotinylated by different methods

  • Easy to use – complete kit includes optimized reagents for binding reactions and RNA probe detection

  • Non-radioactive – eliminate waste concerns from radioactive RNA probes



Studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA)
LightShift RNA EMSA Optimization and Control Kit (20158X), 1 kit; Chemiluminescent Nucleic Acid Detection Module (89880), 1 kit
LightShift Chemiluminescent RNA EMSA Kit

Multi-component kit with reagents for RNA-protein binding reactions and blot detection

100 binding reactions and detection on 1000cm2 of membrane (10 mini-gel blots)

100-rxn kit