Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit
Study RNA-protein interactions with this non-radioactive EMSA kit for gel shift assays.
Manufacturer: Thermo Scientific™ PI20158
Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.
Sensitive – chemiluminescent detection comparable to radioactive detection
Time-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems
Flexible – compatible with RNA probes biotinylated by different methods
Easy to use – complete kit includes optimized reagents for binding reactions and RNA probe detection
Non-radioactive – eliminate waste concerns from radioactive RNA probes
|Studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA)|
|LightShift RNA EMSA Optimization and Control Kit (20158X), 1 kit; Chemiluminescent Nucleic Acid Detection Module (89880), 1 kit|
|LightShift Chemiluminescent RNA EMSA Kit|
Multi-component kit with reagents for RNA-protein binding reactions and blot detection
|100 binding reactions and detection on 1000cm2 of membrane (10 mini-gel blots)|