Laminin B2/γ1 Ab-1 Rat Monoclonal Antibody, Epredia™

Description

Laminins are large heterotrimeric, noncollagenous glycoproteins composed of α, β, and γ chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.

Host Species: Rat

Clone: A5

Isotype: IgG2a/κ

Species Reactivity: Human and Mouse. Others not known.

Epitope: Not determined

Immunogen: Murine EHS laminin preparation

Molecular Weight: ∽210kDa

Positive Control: LS174T cells, Normal colon or colon CA

Cellular Localization: Basement membrane Specificity of Ab-1 was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific MAbs. Ab-1 stains uniformly all human and murine basement membranes.

Recommended for:

• Immunohistochemistry (Acetone-fixed frozen and acid-alcohol-fixed, paraffin-embedded)
• Immunofluorescence
• Immunoprecipitation (Native and denatured)

Note:

USA: RUO; Int'l: RUO