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Thermo Scientific™ Lambda Exonuclease


Lambda Exonuclease is a highly processive exodeoxyribonuclease that selectively digests the 5'-phosphorylated strand of double-stranded DNA in 5'-3' direction.

Manufacturer: thermo scientific™  EN0562

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 Disclaimers

Use of this enzyme in certain applications may be covered by patents and may require a license.

Catalog No. FEREN0562

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Description & Specifications

Specifications

Concentration 10U/µL
Source E.coli cells with a cloned exo gene of phage lambda
Molecular Weight 26kDa monomer
Units 5,000U

Thermo Scientific Lambda Exonuclease is a highly processive 5'=>3' exodeoxyribonuclease. It selectively digests the 5'-phosphorylated strand of double-stranded DNA. The enzyme exhibits low activity on single-stranded DNA and non-phosphorylated DNA, and has no activity at nicks and limited activity at gaps in DNA.

Highlights

  • Active in Thermo Scientific PCR buffers

Applications

  • Generating single-stranded PCR products for use in:
    • DNA sequencing
    • Analysis of DNA single-strand conformation polymorphism (SSCP)
    • Rolling circle amplification
     
  • Producing single-stranded DNA from double-stranded DNA fragments
  • Cloning of PCR products

Note

Use of this enzyme in certain applications may be covered by patents and may require a license.