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KLRG1, PE-eFluor 610, clone: 2F1, eBioscience™

Syrian Hamster Monoclonal Antibody

Manufacturer:  Life Technologies LS61589382

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Catalog No. 501124845



Description

Description

Killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA or 2F1-Ag), is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. Functionally, KLRG1 is a lymphocyte that is involved in the lysis of certain tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.
Specifications

Specifications

KLRG1
Monoclonal
0.2 mg/mL
PBS with 0.09% sodium azide; pH 7.2
O88713
2F1, CLEC15A, MAFA, MAFA-2F1, MAFA-L, MAFA-LIKE, C-type lectin domain family 15 member A, C-type lectin domain family 15, member A, ITIM-containing receptor MAFA-L, MAFA-like receptor, killer cell lectin-like receptor subfamily G member 1, mast cell function-associated antigen (ITIM-containing)
Syrian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
2F1
PE-eFluor® 610
Klrg1
Liquid
Klrg1
IgG
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
50928
Documents

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