Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)

Name: Thermo Scientific&trade; Klenow Fragment, exo&ndash; (5 U/&mu;L)
Brand: Thermo Scientific&trade;
SKU: FEREP0421
Price: 181.00 CAD

Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.

Supplier: Thermo Scientific™ EP0421

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Catalog No. FEREP0421

$181.00 / Each

Quantity

Description

Klenow Fragment, exo-, is the large fragment of DNA polymerase I . It exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities of DNA Polymerase I. The 3'→5' exonuclease activity of the enzyme is eliminated by mutations in the 3'→5'-exonuclease active sit.

Highlights

Lacks 3'→5' exonuclease activity
Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides)
Active in restriction enzyme, PCR, and RT buffers

Applications

Random-primed DNA labeling
Labeling by fill-in 5'-overhangs of dsDNA
Strand displacement amplification (SDA)
DNA sequencing by the Sanger method

Note

Klenow Fragment, exo- is not recommended for DNA blunting reactions prior to DNA ligation since it frequently adds one or more extra nucleotides to the 3'-terminus of blunt-end DNA substrates in a non-template directed fashion

Specifications

Concentration	5 U/μL
Compatible Buffer	Restriction Enzyme, PCR, RT Buffer
Product Type	Klenow Fragment

Polymerase	DNA Polymerase I
Quantity	300 U

For Research Use Only. Not for use in diagnostic procedures.