missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

KIT [V654A] Recombinant Human Protein DFS Item

KIT [V654A] Recombinant Human Protein

Manufacturer:  Life Technologies LSPV4132

 View more versions of this product

Catalog No. PV4132



Description

Description

KIT is a proto-oncogene encoding a transmembrane tyrosine kinase receptor for stem cell factor required for normal hematopoiesis, melangenesis and gametogenesis. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. The clinically observed V654A mutant has been demonstrated to be resistant to inhibition with imatinib.
Specifications

Specifications

NP_000213, V654A
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
53.2kDa
50% by SDS-PAGE
–80°C
1 Each
KIT V654A
KIT V654A was pre-diluted in enzyme dilution buffer and assayed in 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 100μM ATP, 200μg/mL poly [Glu, Tyr] 4:1 substrate and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
PDGFR
KIT [V654A] Recombinant Human Protein
Human
0.28mg/mL
3815, 4775
KIT [V654A] Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Receptor tyrosine kinases
KIT [V654A] Recombinant Human Protein
Kinase assay
Baculovirus
Amino acids 544-976; V654A
6.2nmoles of phosphate transferred to poly [Glu, Tyr] 4:1 substrate per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 20μg/mL
His Tag
Documents

For Research Use Only. Not for use in diagnostic procedures.