Ki-67, eFluor 615, clone: SolA15, eBioscience™
Rat Monoclonal Antibody
Manufacturer: Life Technologies LS42569880
Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with the euchromatin at the perichromosomal layer with centromeric heterochromatin, and with the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution, with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis.Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the Ki-67 labeling index refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin).The SolA15 antibody also recognizes human, non-human primate and canine Ki-67.Applications Reported: This SolA15 antibody has been reported for use in immunocytochemistry and immunohistochemistry.Applications Tested: This SolA15 antibody has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells at less than or equal to 0.5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength. (for example Excitation 560/55, 585LP, Emission 645/75) A standard Alexa Fluor™ 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|antigen identified by monoclonal antibody Ki-67, antigen KI-67, KIA, proliferation-related Ki-67 antigen, protein phosphatase 1, regulatory subunit 105, RP11-380J17.2|
|Canine, Cynomolgus Monkey, Human, Murine, Non-human Primate, Rat|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)|
|IgG2a, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|100686578, 102135895, 17345, 291234, 4288|
For Research Use Only.