Ki-67 Monoclonal Antibody (20Raj1), eFluor™ 660, eBioscience™, Invitrogen™

Description

Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist, a 345 and 395 kDa form that are expressed in dividing cells. Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells. This monoclonal antibody 20Raj1 recognizes canine Ki-67. Applications Reported: This 20Raj1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This 20Raj1 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized HeLa cells, immunohistochemistry on FFPE human skin using low pH antigen retrieval, and flow cytometry on stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. For immunocytochemistry immunohistochemistry, this can be used at less than or equal to 20 μg/mL.

Ki-67 Monoclonal antibody specifically detects Ki-67 in Canine, Human samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin).