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JAK2 [V617F] Recombinant Human Protein, JH1 and JH2 Domains DFS Item

JAK2 [V617F] Recombinant Human Protein, JH1 and JH2 Domains

Manufacturer:  Life Technologies LSPV4336

Catalog No. PV4336



Description

Description

JAK2 is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. This kinase contains both the JH1 (catalytic) and JH2 (pseudokinase) domains of JAK2. The V617F mutation has been shown to be a cause of polycythemia vera.
Specifications

Specifications

NP_004963
Cell viability, proliferation and function, pharma and biopharma, protein assays and analysis, target and lead identification and validation
97.9kDa
70% by SDS-PAGE
–80°C
1 Each
JAK2 JH1 JH2 V617F
JAK2 JH1 JH2 V617F was pre-diluted in enzyme dilution buffer and assayed in 25mM HEPES (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 200μM ATP, 200μg/mL AXLtide peptide substrate (KKSRGDYMTMQIG) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
JAK
JAK2 [V617F] Recombinant Human Protein
Human
0.19mg/mL
3717
JAK2 [V617F] Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Cytoplasmic tyrosine kinase
JAK2 [V617F] Recombinant Human Protein
Kinase assay
Baculovirus
Amino acids 532-1132; V617F
18nmoles of phosphate transferred to AXLtide peptide substrate (KKSRGDYMTMQIG) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 4.17μg/mL
GST Tag
Documents

For Research Use Only. Not for use in diagnostic procedures.