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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli DFS Item


Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing

Manufacturer: invitrogen™  LSK287520

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. K287520

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Description & Specifications

Specifications

Product Type Kit, PCR Cloning for Sequencing
Bacterial/Yeast Strain TOP10
For Use With (Application) Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Cloning, Methylation Analysis, PCR Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Sequencing Bisulfite-Treated DNA
Includes Box 1: Topoisomerase I-activated pCR4Blunt-TOPO vector, Salt solution, dNTPs, Control template, T3, T7, M13 forward, and M13 reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
Quantity 25 reactions