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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Electrocomp™ E. coli DFS Item

Offers the fastest and easiest method for high-efficiency cloning of blunt-end PCR products amplified with proofreading thermostable polymerases

Manufacturer: invitrogen™  K286040

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Catalog No. K286040


Description & Specifications


Product Type Kit, PCR Cloning
Bacterial/Yeast Strain TOP10
Includes Box 1: Linearized and topoisomerase I-activated pCRBlunt II-TOPO vector, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
For Use With (Application) Cloning, PCR Cloning
Quantity 50 reactions

Kit includes linearized and topoisomerase I-activated pCR™-Blunt II-TOPO Vector for 5-minute benchtop ligations without ligase.

  • ccdB gene for positive selection
  • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
  • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli
  • SP6 promoter/primer site for in vitro RNA transcription and sequencing
  • M13 forward and reverse primer sites for sequencing or PCR screening
  • Selected Zero Blunt TOPO PCR Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO-cloned inserts for downstream analysis

Cloning, PCR Cloning