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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells DFS Item


Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

Manufacturer: invitrogen™  K280020

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Catalog No. K280020

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Description & Specifications

Specifications

Product Type Kit, PCR Cloning
Bacterial/Yeast Strain TOP10
Includes Box 1: Linearized and topoisomerase I-activated pCRBlunt II-TOPO vector, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
For Use With (Application) Cloning, PCR Cloning
Quantity 25 reactions