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Invitrogen™ TOPO™ XL PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli DFS Item


Combines a unique vector and novel protocol for easy, efficient cloning of long (3-10kb) PCR products

Manufacturer: invitrogen™  K475020

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Catalog No. K475020

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Description & Specifications

Specifications

Product Type Kit, PCR Cloning
Bacterial/Yeast Strain TOP10
Includes Each TOPO XL PCR Cloning Kit includes linearized and topoisomerase I-activated pCR-XL-TOPO vector, dNTPs, control template and primers, M13 forward and reverse primers, sodium iodide solution, binding buffer, 4X final wash, S.N.A.P. purification columns and collection vials, 6X crystal violet loading buffer, 2mg/mL crystal violet solution, One Shot Electrocomp or Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid
For Use With (Application) Cloning, PCR Cloning
Quantity 20 reactions

Topoisomerase I activation allows PCR products to be ligated in just 5 minutes on your bench top and typically results in >80% recombinants. The kit uses the linearized and topoisomerase I-activated pCR™-XL-TOPO vector.

Novel Protocol
The TOPO XL PCR Cloning Kit protocol includes S.N.A.P.™ gel purification which employs crystal violet for improved efficiency of long PCR cloning. This eliminates the need for ethidium bromide and exposure to UV light, which can damage long PCR products and reduce the number of recombinants.

  • ccdB gene for positive selection
  • EcoR I and Nsi I sites flanking the PCR product insertion site for easy excision of inserts
  • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli
  • T7 promoter/priming site for in vitro RNA transcription and sequencing
  • M13 forward and reverse primer sites for sequencing

Cloning, PCR Cloning