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Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with TOP10F' E. coli DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Manufacturer:  Invitrogen™ LSK455040

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Catalog No. K455040


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications Description & Specifications

Specifications

Kit, Cloning for Subcloning
Box 1: Topoisomerase I-activated pCR2.1-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
50 reactions
TOP10F'
Cloning, PCR Cloning