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Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with TOP10 E. coli, without manual DFS Item


Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

Manufacturer: invitrogen™  LSKNM450040

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. KNM450040

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Description & Specifications

Specifications

Product Type Kit, Cloning for Subcloning
Bacterial/Yeast Strain TOP10
Includes Box 1: Topoisomerase I-activated pCR2.1-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
Quantity 50 reactions
For Use With (Application) Cloning, PCR Cloning