Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Electrocomp™ E. coli
Provides a highly efficient, 5 minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing
Manufacturer: invitrogen™ LSK458001
For Research Use Only. Not for use in diagnostic procedures.
|For Use With (Application)||Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Cloning, Methylation Analysis, PCR Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Sequencing Bisulfite-Treated DNA|
|Product Type||Kit, Cloning for Sequencing|
|Includes||Box 1: Topoisomerase I-activated pCR4-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, T3, T7, M13 forward, and M13 reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid|