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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Electrocomp™ E. coli DFS Item

Provides a highly efficient, 5 minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing

Manufacturer:  Invitrogen™ LSK458001

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Catalog No. K458001


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications Description & Specifications

Specifications

Kit, Cloning for Sequencing
Box 1: Topoisomerase I-activated pCR4-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, T3, T7, M13 forward, and M13 reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
25 reactions
TOP10
Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Cloning, Methylation Analysis, PCR Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Sequencing Bisulfite-Treated DNA