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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli DFS Item


Provides a highly efficient, 5 minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing

Manufacturer: invitrogen™  LSK459501

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. K459501

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Description & Specifications

Specifications

Includes Box 1: Topoisomerase I-activated pCR4-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, T3, T7, M13 forward, and M13 reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
For Use With (Application) Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Cloning, Methylation Analysis, PCR Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Sequencing Bisulfite-Treated DNA
Product Type Kit, Cloning for Sequencing
Quantity 25 reactions
Bacterial/Yeast Strain DH5α™