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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli DFS Item

Provides a highly efficient, 5 minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing

Manufacturer:  Invitrogen™ LSK459501

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Catalog No. K459501


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications Description & Specifications

Specifications

Kit, Cloning for Sequencing
Box 1: Topoisomerase I-activated pCR4-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, T3, T7, M13 forward, and M13 reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
25 reactions
DH5α™
Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Cloning, Methylation Analysis, PCR Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Sequencing Bisulfite-Treated DNA