Please login to your online account to display your discounted pricing

Invitrogen™ TOPO™ TA Cloning™ Kit, with One Shot™ TOP10F' Chemically Competent E. coli, no manual DFS Item

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

$777.00 - $1,354.50

Specifications

Product Type Kit, Cloning
For Use With (Application) Cloning, PCR Cloning
Includes Box 1: Topoisomerase I-activated pCR2.1-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
Quantity 50 reactions
View More Specs
Includes:  Topoisomerase I-activated pCR™2.1-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

KNM455040

DFS Item
 
invitrogen™
KNM455040
50 reactions Each for $1,354.50

KNM455001

DFS Item
 
invitrogen™
KNM455001
25 reactions Each for $777.00
Description & Specifications

Specifications

Product Type Kit, Cloning
For Use With (Application) Cloning, PCR Cloning
Includes Box 1: Topoisomerase I-activated pCR2.1-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
Quantity 50 reactions

Uses a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
  • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
  • Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants
  • TOPO TA Cloning Kits are also available in combo kits combined with a PureLink™Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO-cloned inserts for downstream analysis

Cloning, PCR Cloning