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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCRII-TOPO Vector and One Shot™ TOP10 Electrocomp™ E. coli DFS Item


Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Manufacturer: invitrogen™  K466001

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Catalog No. K466001

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Description & Specifications

Specifications

Product Type Kit, Cloning
Bacterial/Yeast Strain TOP10
Includes Box 1: Topoisomerase I-activated pCRII-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
For Use With (Application) Cloning, PCR Cloning
Quantity 25 reactions

Uses a pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
  • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
  • Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants

Cloning, PCR Cloning