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ThermoScript™ RT-PCR System for First-Strand cDNA Synthesis DFS Item

Optimized for generating first-strand cDNA from total or poly(A)+ RNA

Manufacturer:  Life Technologies LS11146016

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Catalog No. 11146016


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications Description & Specifications

Specifications

First-Strand cDNA Synthesis, RT-PCR
Random Primers, Oligo dT Primers
Store at -20deg.C
50° to 65°C
100μL ThermoScript RT (15U/μL); 500μL 5X cDNA Synthesis Buffer; 250μL 0.1M DTT; 2 × 250μL 10mM dNTP Mix; 100μL RNaseOUT (40U/μL); 100μL Oligo (dT)20 (50μM); 250μL Random Hexamers (50ng/μL); 1.25mL DEPC-Treated Water; 2 × 50μL E. coli Rnase H (2U/μL)
100 reactions
122° to 149°F