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Invitrogen™ ThermoScript™ RT-PCR System for First-Strand cDNA Synthesis DFS Item

Optimized for generating first-strand cDNA from total or poly(A)+ RNA

$402.15 - $1,119.30

Specifications

For Use With (Application) First-Strand cDNA Synthesis, RT-PCR
Primer Random Primers, Oligo dT Primers
Storage Requirements Store at -20deg.C
Temperature (Metric) Operating 50° to 65°C
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Includes Price Quantity    

11146016

DFS Item
 
life technologies
11146016
100μL ThermoScript RT (15U/μL); 500μL 5X cDNA Synthesis Buffer; 250μL 0.1M DTT; 2 × 250μL 10mM dNTP Mix; 100μL RNaseOUT (40U/μL); 100μL Oligo (dT)20 (50μM); 250μL Random Hexamers (50ng/μL); 1.25mL DEPC-Treated Water; 2 × 50μL E. coli Rnase H (2U/μL) Each for $1,119.30

11146024

DFS Item
 
life technologies
11146024
25μL ThermoScript RT (15U/μL); 500μL 5X cDNA Synthesis Buffer; 250μL 0.1M DTT; 100μL 10mM dNTP Mix; 25μL RNaseOUT (40U/μL); 25μL Oligo (dT)20 (50μM); 50μL Random Hexamers (50ng/μL); 1.25mL DEPC-Treated Water; 50μL E. coli RNase H (2U/μL) Each for $402.15
Description & Specifications

Specifications

For Use With (Application) First-Strand cDNA Synthesis, RT-PCR
Primer Random Primers, Oligo dT Primers
Storage Requirements Store at -20deg.C
Temperature (Metric) Operating 50° to 65°C

The ThermoScript RT-PCR System for First-Strand cDNA Synthesis Kit is an avian RT with reduced RNase H activity designed for RT-PCR of the most highly structured, difficult RNA templates. The ThermoScript RT-PCR systems are designed to copy RNA with secondary structure and to improve specificity of priming. In conjunction with PCR, the system can be used to detect the presence of rare messages or to quantitate the amount of specific mRNA from small numbers of cells. The systems contain ThermoScript RT, a cloned avian reverse transcriptase that has been engineered to have reduced RNase H activity and to have high thermal stability (up to 70°C).

  • Optimized for RT-PCR
  • Can be used with highly structured RNA or gene-specific primers
  • Reduces RNase H activity for greater first-strand cDNA yield
  • Amplifies first-strand cDNA products directly without organic extractions or precipitations
  • Includes oligo(dT) and random hexamers to prime cDNA synthesis for flexible use
  • Offers a choice of Platinum Taq DNA Polymerase for higher specificity when amplifying templates up to 3kb, or Platinum Taq DNA Polymerase High Fidelity for amplifying templates up to 12kb
  • Optimal reaction temperature: 50° to 65°C

PCR & Real-Time PCR, Reverse Transcription

Shipping Condition: Dry ice