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ThermoScript™ RT-PCR System for First-Strand cDNA Synthesis DFS Item


Optimized for generating first-strand cDNA from total or poly(A)+ RNA

Manufacturer: life technologies  LS11146024

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 11146024

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Description & Specifications

Specifications

Includes 25μL ThermoScript RT (15U/μL); 500μL 5X cDNA Synthesis Buffer; 250μL 0.1M DTT; 100μL 10mM dNTP Mix; 25μL RNaseOUT (40U/μL); 25μL Oligo (dT)20 (50μM); 50μL Random Hexamers (50ng/μL); 1.25mL DEPC-Treated Water; 50μL E. coli RNase H (2U/μL)
Primer Random Primers, Oligo dT Primers
Temperature (English) Operating 122° to 149°F
For Use With (Application) First-Strand cDNA Synthesis, RT-PCR
Storage Requirements Store at -20deg.C
Quantity 25 reactions
Temperature (Metric) Operating 50° to 65°C