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ThermoScript™ RT-PCR System for First-Strand cDNA Synthesis DFS Item


Optimized for generating first-strand cDNA from total or poly(A)+ RNA

Manufacturer: life technologies  11146024

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Catalog No. 11146024

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Description & Specifications

Specifications

For Use With (Application) First-Strand cDNA Synthesis, RT-PCR
Includes 25μL ThermoScript RT (15U/μL); 500μL 5X cDNA Synthesis Buffer; 250μL 0.1M DTT; 100μL 10mM dNTP Mix; 25μL RNaseOUT (40U/μL); 25μL Oligo (dT)20 (50μM); 50μL Random Hexamers (50ng/μL); 1.25mL DEPC-Treated Water; 50μL E. coli RNase H (2U/μL)
Primer Random Primers, Oligo dT Primers
Quantity 25 reactions
Storage Requirements Store at -20deg.C
Temperature (English) Operating 122° to 149°F
Temperature (Metric) Operating 50° to 65°C

The ThermoScript RT-PCR System for First-Strand cDNA Synthesis Kit is an avian RT with reduced RNase H activity designed for RT-PCR of the most highly structured, difficult RNA templates. The ThermoScript RT-PCR systems are designed to copy RNA with secondary structure and to improve specificity of priming. In conjunction with PCR, the system can be used to detect the presence of rare messages or to quantitate the amount of specific mRNA from small numbers of cells. The systems contain ThermoScript RT, a cloned avian reverse transcriptase that has been engineered to have reduced RNase H activity and to have high thermal stability (up to 70°C).

  • Optimized for RT-PCR
  • Can be used with highly structured RNA or gene-specific primers
  • Reduces RNase H activity for greater first-strand cDNA yield
  • Amplifies first-strand cDNA products directly without organic extractions or precipitations
  • Includes oligo(dT) and random hexamers to prime cDNA synthesis for flexible use
  • Offers a choice of Platinum Taq DNA Polymerase for higher specificity when amplifying templates up to 3kb, or Platinum Taq DNA Polymerase High Fidelity for amplifying templates up to 12kb
  • Optimal reaction temperature: 50° to 65°C

PCR & Real-Time PCR, Reverse Transcription