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ThermoScript™ Reverse Transcriptase DFS Item

An avian RT with reduced RNase H activity designed for RT-PCR

Manufacturer: life technologies  12236014

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Catalog No. 12236014


Description & Specifications


Concentration 15U/μL
Format Frozen
Includes 5X cDNA synthesis buffer (250mM Tris acetate (pH 8.4), 375mM potassium acetate, 40mM magnesium acetate, and stabilizers), and 100mM DTT
Packaging Type Tube
Quantity 25 reactions (375 units)
Storage Requirements Store at -70deg.C
Temperature (English) Operating 149°F
Temperature (Metric) Operating 65°C
Test Sensitivity Medium

ThermoScript Reverse Transcriptase (RT) is engineered to have high thermal stability, copy RNA with secondary structure, and improve priming specificity.

  • Yield—Reduced RNase H activity results in greater first-strand cDNA yields
  • Size of cDNA—Generation of RT-PCR products up to 12kb
  • Thermostability—ThermoScript Reverse Transcriptase is active up to 70°C
  • Flexibility—Uses either total or poly(A)+ RNA
  • Concentration—15U/μL
  • Unit Definition: One unit incorporates 1nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)-oligo(dT)25 as template-primer

  • Optimal reaction temperature: 65°C
  • Final product size: 12.3kb or less

PCR & Real-Time PCR, Reverse Transcription