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Invitrogen™ ThermoScript™ Reverse Transcriptase DFS Item

An avian RT with reduced RNase H activity designed for RT-PCR

$388.50 - $1,155.00

Specifications

Includes 5X cDNA synthesis buffer (250mM Tris acetate (pH 8.4), 375mM potassium acetate, 40mM magnesium acetate, and stabilizers), and 100mM DTT
Storage Requirements Store at -70deg.C
Temperature (Metric) Operating 65°C
Test Sensitivity Medium
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 Disclaimers

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

12236014

DFS Item
 
life technologies
12236014
25 reactions (375 units) Each for $388.50

12236022

DFS Item
 
life technologies
12236022
100 reactions (1500 units) Each for $1,155.00
Description & Specifications

Specifications

Includes 5X cDNA synthesis buffer (250mM Tris acetate (pH 8.4), 375mM potassium acetate, 40mM magnesium acetate, and stabilizers), and 100mM DTT
Storage Requirements Store at -70deg.C
Temperature (Metric) Operating 65°C
Test Sensitivity Medium

ThermoScript Reverse Transcriptase (RT) is engineered to have high thermal stability, copy RNA with secondary structure, and improve priming specificity.

  • Yield—Reduced RNase H activity results in greater first-strand cDNA yields
  • Size of cDNA—Generation of RT-PCR products up to 12kb
  • Thermostability—ThermoScript Reverse Transcriptase is active up to 70°C
  • Flexibility—Uses either total or poly(A)+ RNA
  • Concentration—15U/μL
  • Unit Definition: One unit incorporates 1nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)-oligo(dT)25 as template-primer

  • Optimal reaction temperature: 65°C
  • Final product size: 12.3kb or less

PCR & Real-Time PCR, Reverse Transcription

Shipping Condition: Dry ice