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Invitrogen™ ThermoScript™ Reverse Transcriptase DFS Item

An avian RT with reduced RNase H activity designed for RT-PCR

$401.00 - $1,198.00

Specifications

Includes 5X cDNA synthesis buffer (250mM Tris acetate (pH 8.4), 375mM potassium acetate, 40mM magnesium acetate, and stabilizers), and 100mM DTT
Storage Requirements Store at -70deg.C
Temperature (Metric) Operating 65°C
Test Sensitivity Medium
View More Specs

Disclaimers:

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.



Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
12236014 Life Technologies
LS12236014
25 reactions (375 units) Each for $401.00
12236022 Life Technologies
LS12236022
100 reactions (1500 units) Each for $1,198.00
Specifications

Description

ThermoScript Reverse Transcriptase (RT) is engineered to have high thermal stability, copy RNA with secondary structure, and improve priming specificity.

  • Yield—Reduced RNase H activity results in greater first-strand cDNA yields
  • Size of cDNA—Generation of RT-PCR products up to 12kb
  • Thermostability—ThermoScript Reverse Transcriptase is active up to 70°C
  • Flexibility—Uses either total or poly(A)+ RNA
  • Concentration—15U/μL
  • Unit Definition: One unit incorporates 1nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)-oligo(dT)25 as template-primer

  • Optimal reaction temperature: 65°C
  • Final product size: 12.3kb or less

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Description & Specifications

Specifications

5X cDNA synthesis buffer (250mM Tris acetate (pH 8.4), 375mM potassium acetate, 40mM magnesium acetate, and stabilizers), and 100mM DTT
Store at -70deg.C
65°C
Medium