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Invitrogen™ TA Cloning™ Kits with pCR™ 2.1 Vector, without Competent Cells DFS Item

Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

Manufacturer: invitrogen™  K202020

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Catalog No. K202020


Description & Specifications


Bacterial Content INVαF'
Includes TA Cloning kits contain linearized pCR2.1 vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls.
Quantity 20 reactions
Storage Requirements Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Vector TA Cloning Vectors

The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

  • Fast and convenient: 15-minute, room-temperature ligation
  • Efficient: blue/white screening and >80% clones with correct insert
  • Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
  • Hassle-free: eliminates any enzymatic modifications of the PCR product
  • Streamlined: does not require the use of PCR primers that contain restriction sites

  • Provides 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 promoter for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of insert
  • M13 forward and reverse primer sites for sequencing
  • How TA Cloning Works
  • Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
  • Linearized vector supplied in this kit has single 3' deoxythymidine (T) residues
  • Allows PCR inserts to ligate efficiently with vector

Cloning, PCR Cloning