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Invitrogen™ TA Cloning™ Kits, Dual Promoter with pCR™ 2.1 Vector and One Shot™ Chemically Competent Cells DFS Item


Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector

Manufacturer: invitrogen™  LSK206040

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. K206040

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Description & Specifications

Specifications

Bacterial Content TOP10
Product Type One Shot™ TOP10F' chemically competent E. coli
Quantity 40 reactions
Vector Blunt DNA Cloning Vectors
Includes Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot TOP10F' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
Storage Requirements Store One Shot E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Product Name One Shot™ TOP10F' chemically competent E. coli
Quantity 40 reactions