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Invitrogen™ TA Cloning™ Kits, Dual Promoter with pCR™ 2.1 Vector and One Shot™ Chemically Competent Cells DFS Item

Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector

Manufacturer: invitrogen™  K206001

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Catalog No. K206001


Description & Specifications


Bacterial Content Not Included
Includes Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot TOP10F' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
Product Type One Shot™ TOP10F' chemically competent E. coli
Quantity 20 reactions
Storage Requirements Store One Shot E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Vector TA Cloning Vectors

The T7 and Sp6 promoters of the pCRII vector allow in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCRII cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

  • Fast and convenient: 15-minute, room-temperature ligation
  • Efficient: blue/white screening and >80% clones with correct insert
  • Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
  • Hassle-free: eliminates any enzymatic modifications of PCR product
  • Streamlined: does not require use of PCR primers that contain restriction sites
  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 and Sp6 promoters for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of inserts
  • M13 forward and reverse primer sites for sequencing

  • How TA Cloning Works\
  • Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
  • Linearized vector supplied in this kit has single 3' deoxythymidine (T) residues
  • Allows PCR inserts to ligate efficiently with vector
  • Kit Configurations
  • TA Cloning Kit is offered in a variety of configurations: with One Shot INVF' Chemically Competent E. coli (K2050-01 and K2050-40), One Shot TOP10F' Chemically Competent E. coli (K2060-01 and K2060-40), and without competent cells (K2750-20 and K2750-40) in 20- and 40-reaction kit sizes

Cloning, PCR Cloning