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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10F' Chemically Competent E. coli DFS Item


Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector

Manufacturer: invitrogen™  K203001

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Catalog No. K203001

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Description & Specifications

Specifications

Bacterial Content Not Included
Includes Linearized pCR2.1 vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot TOP10F' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
Product Type One Shot™ TOP10F' chemically competent E. coli
Quantity 20 reactions
Storage Requirements Store One Shot E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Vector TA Cloning Vectors

The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

  • Fast and convenient: 15-minute, room-temperature ligation
  • Efficient: blue/white screening and >80% clones with correct insert
  • Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
  • Hassle-free: eliminates any enzymatic modifications of PCR product
  • Streamlined: does not require use of PCR primers that contain restriction sites
  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 promoter for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of inserts
  • M13 forward and reverse primer sites for sequencing

  • How TA Cloning™ Works
  • Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
  • Llinearized vector supplied in this kit has single 3' deoxythymidine (T) residues
  • Allows PCR inserts to ligate efficiently with vector
  • Kit Configurations
  • TA Cloning™ Kit is offered in a variety of configurations: without competent cells (K2020-20 and K2020-40), with One Shot™ INVF' Chemically Competent E. coli (K2000-01 and K2000-40), with One Shot™ TOP10F' Chemically Competent E. coli (K2030-01 and K2030-40), and with One Shot™ TOP10 Chemically Competent E. coli (K2040-01 and K2040-40) in 20- and 40-reaction kit sizes

Cloning, PCR Cloning