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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli, no manual DFS Item

Designed for cloning Taq-amplified PCR products directly from PCR reaction using overnight ligation step

Manufacturer: invitrogen™  KNM204001

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Catalog No. KNM204001

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Description & Specifications


Bacterial Content TOP10
Includes Linearized pCR2.1vector, T4 DNA ligase, 10X ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls, One Shot TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Without manual.
Product Type One Shot TOP10 Chemically Competent E. coli
Quantity 20 reactions
Storage Requirements Store One Shot E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Vector TA Cloning Vectors

TA Cloning Kits use a pCR vector and yield >=80% recombinants with >=90% of the recombinants containing inserts.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • Choice of T7 (pCR2.1) or T7 and Sp6 (pCRII) promoters for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of inserts
  • Choice of kanamycin or ampicillin selection
  • Easy blue/white colony screening
  • M13 forward and reverse primer sites for sequencing
  • Manual not included
  • Cloning Method: Restriction enzyme/MCS

Cloning, PCR Cloning