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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli, no manual DFS Item

Designed for cloning Taq-amplified PCR products directly from PCR reaction using overnight ligation step

$751.80 - $1,304.10

Specifications

Includes Linearized pCR2.1vector, T4 DNA ligase, 10X ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls, One Shot TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Without manual.
Storage Requirements Store One Shot E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Vector TA Cloning Vectors
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.



Products ${productFamilyLength}
Catalog Number Mfr. No. Bacterial Content Quantity Price Quantity  
Catalog Number Mfr. No. Bacterial Content Quantity Price Quantity  
KNM204001 Invitrogen™
LSKNM204001
TOP10 20 reactions Each for $751.80
KNM204040 Invitrogen™
LSKNM204040
INVαF' 40 reactions Each for $1,304.10
Specifications

Description

TA Cloning Kits use a pCR vector and yield >=80% recombinants with >=90% of the recombinants containing inserts.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • Choice of T7 (pCR2.1) or T7 and Sp6 (pCRII) promoters for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of inserts
  • Choice of kanamycin or ampicillin selection
  • Easy blue/white colony screening
  • M13 forward and reverse primer sites for sequencing
  • Manual not included
  • Cloning Method: Restriction enzyme/MCS

Cloning, PCR Cloning

Description & Specifications

Specifications

Linearized pCR2.1vector, T4 DNA ligase, 10X ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls, One Shot TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Without manual.
Store One Shot E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
TA Cloning Vectors