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Invitrogen™ T4 Polynucleotide Kinase DFS Item

Catalyzes transfer of γ-phosphate of ATP to 5'hydroxyl terminus of DNA or RNA

$197.40 - $741.30

Specifications

Storage Requirements Store at -20°C
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Includes:  A vial of 5X forward reaction buffer [350mM Tris-HCl (pH 7.6), 50mM MgCl2, 500mM KCl, 5mM 2-mercaptoethanol] and a vial of 5X exchange reaction buffer [250mM imidazole-HCl (pH 6.4), 60mM MgCl2, 5mM 2-mercaptoethanol, 350μM ADP]

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

18004028

DFS Item
 
life technologies
18004028
1000 units Each for $741.30

18004010

DFS Item
 
life technologies
18004010
200 units Each for $197.40
Description & Specifications

Specifications

Storage Requirements Store at -20°C

  • Source: Purified from E. coli expressing the T4 polynucleotide kinase gene in a λ vector
  • Performance and Quality Testing: Ribonuclease, endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease, and phosphatase assays; labeling efficiency tested
  • Unit Definition: One unit transfers 1nmol of phosphate of ATP to the 5' hydroxyl termini of micrococcal nuclease-treated DNA in 30 min. at 37°C
  • Unit Reaction Conditions: 70mM Tris-HCl (pH 7.6), 0.1M KCl, 10mM MgCl2 , 1mM 2-mercaptoethanol, 0.3mM [γ-32P]ATP, 0.38mg/mL micrococcal nuclease-treated DNA, and enzyme in 150μL for 30 min. at 37°C

5' end-labeling of DNA or RNA, Phosphorylation of synthetic linkers, Cloning, DNA & RNA Purification & Analysis, DNA Labeling, Nucleic Acid Labeling & Oligo Synthesis, Oligo Labeling, Restriction Enzyme Cloning

Shipping Conditions: Approved for shipment on wet or dry ice