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SuperScript™ Double-Stranded cDNA Synthesis Kit DFS Item


Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis

Manufacturer: life technologies  11917010

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Primer

Catalog No. 11917010

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Description & Specifications

Specifications

Product Type Kit, cDNA Synthesis
Includes The kit includes reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50μg total RNA or 0.2-5μg mRNA into double-stranded cDNA
For Use With (Application) DNA and RNA Purification and Analysis, DNA Labeling, Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis, PCR and Real-Time PCR, Reverse Transcription
Quantity 10 reactions

Contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality, double-stranded cDNA from total RNA or poly A+-selected RNA (mRNA).

Simple, reliable, and trouble-free
The SuperScript Double-Stranded cDNA Synthesis Kit offers a simple, reliable, and trouble-free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments. The reactions described in the SuperScript Double-Stranded cDNA Synthesis Kit protocol are designed to convert 25–50μg total RNA, or 0.2–5μg mRNA, into first- and second-strand cDNA (supply your own primer). Enjoy the benefits of a tested protocol and reagents of consistent quality. To get the best possible cDNA product using this kit, use a high-quality RNA substrate.

  • Reliability: each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologies
  • Performance: SuperScript II is an enzyme you can trust for high-yield, high-quality, general-purpose, first-strand synthesis
  • Optimal temperature: 42°C

DNA and RNA Purification and Analysis, DNA Labeling, Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis, PCR and Real-Time PCR, Reverse Transcription