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Invitrogen™ PureLink™ HiPure Plasmid Miniprep Kit DFS Item


Highly-quality purified plasmid DNA in under two hours

Manufacturer: invitrogen™  K210002

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Catalog No. K210002

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Description & Specifications

Specifications

For Use With (Application) Cloning, In Vitro Transcription, Next-Generation Sequencing, Nucleic Acid Labeling, PCR, Sequencing, Transfection, Transformation
Includes
  • 10mL Resuspension Buffer (R3)
  • 100 μl RNase A
  • 10mL Lysis Buffer (L7)
  • 10mL Precipitation Buffer (N3)
  • 50mL Equilibration Buffer (EQ1)
  • 125mL Wash Buffer (W8)
  • 25mL Elution Buffer (E4)
  • 15mL TE Buffer
  • 25 HiPure Columns
Isolation Technology Silica Spin Column
Quantity 25 preps
Sample Size < 100 μg (Small-Scale) Plasmid DNA
Sample Type Bacterial Culture
Storage Requirements Store all components at room temperature.
Target BAC DNA, Plasmid DNA

The PureLink HiPure Plasmid Miniprep Kit allows isolation of high yields of highly pure plasmid DNA from E.coli in less than 2 hours, without use of any organic solvents or cesium chloride, and contains Low endotoxin levels.

Plasmid DNA of High Purity

  • PureLink HiPure Plasmid Miniprep Kit is designed to efficiently isolate plasmid DNA from E.coli in 1.5 - 2 hours
  • Kit employs anion-exchange resin composed of small particles with a uniform pore size to purify plasmid DNA to a level equivalent to two passes through CsCl gradients
  • Need for additional steps to remove contaminants such as phenol, chloroform, and CsCl are eliminated, minimizing exposure to and disposal of hazardous materials
  • Efficient – Increased yields with low endotoxin Levels
High Yield and Efficient Endotoxin Removal
  • PureLink HiPure Plasmid Miniprep Kit allows purification of up to 30μg of high quality plasmid DNA from 1-3mL overnight E.coli cultures in approximately 1 hour, with an endotoxin level of 0.1-1 EU/μg, when cloning high copy number plasmids
  • Typically, DNA isolated using the PureLink HiPure Plasmid Miniprep Kit has an A260/A280 >1.80 when samples are diluted in Tris-HCl (pH 7.5) indicating that the DNA is reasonably free of proteins that could interfere with downstream applications
Isolated DNA can be used for a Number of Downstream Applications:
  • Purified DNA is suitable for downstream applications, including those requiring the highest purity DNA, such as mammalian transfections, automated and manual DNA sequencing, PCR amplification, in vitro transcription, bacterial cell transformation, cloning, and labeling

DNA & RNA Purification & Analysis, DNA Extraction, Miniprep, Plasmid DNA Purification