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Invitrogen™ PureLink™ HiPure Plasmid Maxiprep Kit DFS Item


No organic solvents, ethidium bromide, or cesium chloride

Manufacturer: invitrogen™  K210007

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Catalog No. K210007

  • / Each
 

Description & Specifications

Specifications

For Use With (Application) Cloning, In Vitro Transcription, Next-Generation Sequencing, Nucleic Acid Labeling, PCR, Sequencing, Transfection, Transformation
Includes
  • 250mL Resuspension Buffer (R3)
  • 1.5mL RNase A
  • 250mL Lysis Buffer (L7)
  • 250mL Precipitation Buffer (N3)
  • 2 × 400mL Equilibration Buffer (EQ1)
  • 3 × 500mL Wash Buffer (W8)
  • 400mL Elution Buffer (E4)
  • 30mL TE Buffer
  • 25 HiPure Columns
  • 5 Column Holders
Isolation Technology Anion Exchange Resin with Lysate Clearing Filter
Quantity 25 preps
Sample Size > 200 μg (Large-Scale) Plasmid DNA
Sample Type Bacterial Culture
Storage Requirements Store all components at room temperature.
Target BAC DNA, Plasmid DNA
Yield 1000 μg

The PureLink HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Maxiprep Kit protocol will typically yield 500–850μg plasmid DNA from 100–200mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.

  • Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
  • High-purity, low-endotoxin product- pure enough for mammalian cell transfection
  • Versatility- purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs
Anion-exchange chromatography for plasmid purification
  • PureLink HiPure Plasmid DNA Purification Kits use patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients
  • Resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal
  • Plasmid preparations can typically be completed in less than 2 hours

Contaminant-free DNA

  • Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
  • Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
  • Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection
  • Purification time is two hours

DNA & RNA Purification & Analysis, DNA Extraction, Maxiprep, Plasmid DNA Purification