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Platinum™ SYBR™ GreenER™ qPCR SuperMix Universal DFS Item


Incorporate high-performance technology to provide reliable gene expression data on a several platforms, including ABI 7500, Corbett Rotor-Gene™, MJ Chromo4™ and Opticon™, and Stratagene instruments

Manufacturer: life technologies  11762500

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Catalog No. 11762500

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Description & Specifications

Specifications

For Use With (Application) 7500 System, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Includes 2X SYBR GreenER qPCR SuperMix and a separate tube of ROX reference dye
No. of Reactions 500.0

Eight cDNA targets and eight genomic DNA targets were amplified from 1ng of cDNA and 6pg of gDNA, respectively, on the ABI PRISM™ 7900HT Sequence Detection System using the SYBR GreenER qPCR SuperMix for ABI PRISM instruments. For all 16 targets, detection was positive in the presence of template but negative for all no-template controls.

The SYBR GreenER system is specially formulated to offer the best sensitivity, specificity, and reproducibility with:

  • A novel dsDNA-binding dye that exhibits a brighter signal for increased sensitivity and less PCR inhibition than original SYBR Green I, while using the same instrument filters and settings
  • An optimized buffer system that improves sensitivity and provides excellent long-term stability
  • Uracil DNA glycosylase that reduces carryover contamination in qPCR

  • Novel Formulation Provides More Reliable Data The SYBR GreenER qPCR SuperMix kits include a new dsDNA-binding dye that produces a brighter signal and significantly less PCR inhibition, for improved qPCR performance over a broad dynamic range
  • Consistent Specificity and Low-Copy Detection
  • The SYBR GreenER qPCR reagent system minimizes the formation of nonspecific products, including primer-dimers, for greater accuracy across a wide range of targets. The reliability of the SYBR GreenER qPCR reagent system also translates to greater sensitivity. SYBR GreenER qPCR SuperMix consistently detects fewer than 10 copies of a target in genomic DNA

Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Gene Expression Analysis & Genotyping, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling

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