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Platinum™ SuperScript™ III One-Step RT-PCR System with Platinum Taq High Fidelity DNA Polymerase DFS Item


Offers highest sensitivity for end point detection

Manufacturer: life technologies  12574030

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Catalog No. 12574030

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Description & Specifications

Specifications

Detection Method Gel Electrophoresis
For Use With (Application) RT-PCR
Includes 25μL SuperScript III RT/Platinum Taq High Fidelity Enzyme Mix; 1mL 2X Reaction Mix (containing 0.4mM of each dNTP, 2.4mM MgSO4); 500μL 5mM Magnesium Sulfate
Quantity 25 reactions
Sample Type RNA
Storage Requirements Store all components at -30deg. to -10deg.C.

High-Fidelity PCR Amplification
Platinum Taq DNA Polymerase High Fidelity is ideal for the amplification of DNA fragments, when high yields and robust amplification are required. Fidelity is maximized through the use of a mixture of Platinum Taq DNA Polymerase and the proofreading (3'-5' exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum automatic “hot-start” technology.

  • The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase combines the high thermostability of SuperScript III RT with the specificity of Platinum Taq DNA Polymerase High Fidelity to increase priming specificity, generate higher product yields, and identify a larger range of targets
  • The one-step format enables easy analysis of gene expression or detection of rare or viral RNA.
  • Sensitivity: Routine detection down to 1pg total RNA
  • Convenience: Single-tube format for speed, convenience, and less reaction-to-reaction variability. Compatible with multiplexing
  • Specificity: Uses SuperScript III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers
  • Amplicon Size: Detection of targets from 300 bp to 10kb for greater flexibility

Using the SuperScript One-Step System The system uses a mixture of SuperScript III Reverse Transcriptase and Platinum Taq DNA Polymerase High Fidelity for enhanced RT-PCR yields and fidelity, as well as the detection of longer templates. The system can detect a wide range of RNA targets from 300 bp to 10kb, and is compatible with multiplex applications. The amount of starting material can range from 1pg to 1μg of total RNA. The system consists of two major components: SuperScript III RT/ Platinum Taq High Fidelity Enzyme Mix and 2X Reaction Mix. SuperScript III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45° to 60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. Final product size: 10kb or less.

One-Step RT-PCR, PCR & Real-Time PCR, RT-PCR, Reverse Transcription